1. Bo Carter - Cigarette Blues
2. Blind Boy Fuller - Truckin' My Blues Away No. 2 (Take 2)
3. Big Bill Broozy - Hokum Stomp
4. Bessie Smith - Need a Little Sugar in My Bowl
5. Blind Blake - Too Tight Blues No. 2
6. Rufus & Ben Quillian - Satisfaction Blues
7. Charley Jordan - Keep It Clean
8. Lucille Bogan - Barbecue Bess
9. The Allen Brothers - Bow Wow Blues
10. Dallas String Band - Hokum Blues (Feat. Coley Jones)
11. Kansas Joe - What's the Matter With the Mill? (Feat. Memphis Minnie)
12. Bogus Ben Covington - Adam and Eve in the Garden
13. Barbecue Bob - Honey You're Going Too Fast
14. Papa Charlie Jackson - Shake That Thing
15. Lil Johnson - Sam the Hot Dog Man
16. Charley Patton - Shake It and Break It (But Don't Let It Fall Mama)
17. Noah Lewis' Jug Band - Selling the Jelly
18. Ma Rainey - Ma Rainey's Black Bottom
19. Casey Bill Weldon - Your Wagon's Worn Out Now
20. Frankie 'Half-Pint' Jaxon - Can't You Wait Till You Get Home
21. Mississippi John Hurt - Candy Man Blues
22. Blind Willie McTell - Warm It Up to Me
23. Blind Lemon Jefferson - Black Snake Moan
24. Tampa Red - It's Tight Like That (Feat. Georgia Tom)
25. Walter Coleman - Mama Let Me Lay It On You